Soluble DHFR expression stages in molecules per cell calculated from lysate activity assays as explained in resources and techniques.
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to investigate the constraints imposed by Lon about the DHFR mutational landscape in structural element, we outlined a ∆selection coefficient for each amino acid residue at each posture given that the distinction between the +Lon and –Lon options (determine 3A). The ∆range coefficient values were being most detrimental at positions from the useful category and at positions with a local VILMWF or Y amino acid residue (Figure 3B, excludes Intolerant positions from –Lon collection); Total, mutations at positions with native hydrophobic residues are enriched for destructive ∆selection coefficients (Figure three—determine nutritional supplement 1A).
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